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schneider s s2 medium  (Thermo Fisher)


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    Thermo Fisher schneider s s2 medium
    Schneider S S2 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/schneider+s+s2+medium/SUCROSE+EP%2FBP%2FNF+12KG/pmc13086492-147-8-14
    Average 99 stars, based on 1 article reviews
    schneider s s2 medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes
    Article Snippet: S2R+ cells were grown in serum-supplemented (10%) Schneider's S2 medium (serum SAFC 12103C; media Gibco 21720) at 25 °C.

    Transfection:

    Article Title: Drosophila Activated Cdc42 Kinase Has an Anti-Apoptotic Function
    Article Snippet: .. Schneider 2 (S2) Drosophila cells were transfected with pMT/V5-6xHis-Flag-Ack and pCoHygro in Schneider's S2 medium (Invitrogen). .. Schneider 2 (S2) Drosophila cells were transfected with pMT/V5-6xHis-Flag-Ack and pCoHygro in Schneider's S2 medium (Invitrogen).

    Article Title: The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism
    Article Snippet: .. Drosophila S2 cells were maintained in standard Schneider's S2 medium with fetal bovine serum (Gibco) at 25°C, and transfections were performed using Effectene transfection reagent (Qiagen). ..

    Cell Culture:

    Article Title: ERK inhibits Capicua repressor function via multisite phosphorylation
    Article Snippet: .. S2 cells were cultured at 25°C in standard Schneider's S2 medium with 10% FBS (Gibco) and 5% Pen-Strep (Invitrogen). .. Proteins were induced with 0.35 mM CuSO 4 overnight, cells were lysed in default lysis buffer as above and protein complexes were isolated using anti-V5 beads (A7345, Sigma).



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    https://www.bioz.com/product/schneider+s+s2+medium/SUCROSE+EP%2FBP%2FNF+12KG/pmc13086492-147-8-14
    Average 99 stars, based on 1 article reviews
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    schneider s medium  (Thermo Fisher)
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    schneider ́s drosophila medium  (Thermo Fisher)
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    Fig. 6 Msk is required for Yki nuclear accumulation and Yki-induced organ growth in vivo. a, b Confocal images of <t>Drosophila</t> imaginal discs overexpressing GFP, GFP-Yki, and GFP-Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4). Quantification in the graph (b). From left to right, N = 7, 18, and 22 discs. Scale bar 100 μm. P-value = 2.870e−07 (column 1 vs. 2), 2.392e−06 (column 2 vs. 3) and 0.084 (column 1 vs. 3). c, d Confocal images of Drosophila imaginal discs overexpressing Yki and Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment. Quantification of Yki nucleo-cytoplasmic ratio is shown in graph (d). Zooms are shown below. N = 66 and 54 cells from a representative experiment of 3 independent experiments. Scale bar 10 μm. P-value = 1.007e−14. e Confocal images of Drosophila imaginal discs overexpressing Yki and stained against Yki and Msk. Zooms are shown below. Data representative from 3 biologically independent experiments. Scale bar 10 μm. f Confocal images of Drosophila imaginal discs overexpressing YFP-msk under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4) and stained against Yki and Msk. Data representative of three biologically independent experiments. Scale bar 10 μm. g, h S2 cells overexpressing different combinations of the indicated proteins were grown on top of a ConA coated surface. YFP-Msk was immunoprecipitated with anti- GFP. The immunopurified complexes and total cell lysates (WCL) were immunoblotted as indicated. IgG was marked with an asterisk and Yki was marked with a #. Quantification from three biologically independent co-immunoprecipitations is shown (h). Statistical analysis with a two-tailed unpaired t-test. Data represent mean ± s.e.m. ns non-significant. P-value = 0.167 (4th vs. 5th bar), 0.00023 (4th vs. 6th bar) and 0.0029 (5th vs. 6th bar). Raw data are available in the Source Data file. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.
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    schneider s s2 cell medium  (Thermo Fisher)
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    Fig. 6 Msk is required for Yki nuclear accumulation and Yki-induced organ growth in vivo. a, b Confocal images of <t>Drosophila</t> imaginal discs overexpressing GFP, GFP-Yki, and GFP-Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4). Quantification in the graph (b). From left to right, N = 7, 18, and 22 discs. Scale bar 100 μm. P-value = 2.870e−07 (column 1 vs. 2), 2.392e−06 (column 2 vs. 3) and 0.084 (column 1 vs. 3). c, d Confocal images of Drosophila imaginal discs overexpressing Yki and Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment. Quantification of Yki nucleo-cytoplasmic ratio is shown in graph (d). Zooms are shown below. N = 66 and 54 cells from a representative experiment of 3 independent experiments. Scale bar 10 μm. P-value = 1.007e−14. e Confocal images of Drosophila imaginal discs overexpressing Yki and stained against Yki and Msk. Zooms are shown below. Data representative from 3 biologically independent experiments. Scale bar 10 μm. f Confocal images of Drosophila imaginal discs overexpressing YFP-msk under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4) and stained against Yki and Msk. Data representative of three biologically independent experiments. Scale bar 10 μm. g, h S2 cells overexpressing different combinations of the indicated proteins were grown on top of a ConA coated surface. YFP-Msk was immunoprecipitated with anti- GFP. The immunopurified complexes and total cell lysates (WCL) were immunoblotted as indicated. IgG was marked with an asterisk and Yki was marked with a #. Quantification from three biologically independent co-immunoprecipitations is shown (h). Statistical analysis with a two-tailed unpaired t-test. Data represent mean ± s.e.m. ns non-significant. P-value = 0.167 (4th vs. 5th bar), 0.00023 (4th vs. 6th bar) and 0.0029 (5th vs. 6th bar). Raw data are available in the Source Data file. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.
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    Image Search Results


    Fig. 6 Msk is required for Yki nuclear accumulation and Yki-induced organ growth in vivo. a, b Confocal images of Drosophila imaginal discs overexpressing GFP, GFP-Yki, and GFP-Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4). Quantification in the graph (b). From left to right, N = 7, 18, and 22 discs. Scale bar 100 μm. P-value = 2.870e−07 (column 1 vs. 2), 2.392e−06 (column 2 vs. 3) and 0.084 (column 1 vs. 3). c, d Confocal images of Drosophila imaginal discs overexpressing Yki and Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment. Quantification of Yki nucleo-cytoplasmic ratio is shown in graph (d). Zooms are shown below. N = 66 and 54 cells from a representative experiment of 3 independent experiments. Scale bar 10 μm. P-value = 1.007e−14. e Confocal images of Drosophila imaginal discs overexpressing Yki and stained against Yki and Msk. Zooms are shown below. Data representative from 3 biologically independent experiments. Scale bar 10 μm. f Confocal images of Drosophila imaginal discs overexpressing YFP-msk under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4) and stained against Yki and Msk. Data representative of three biologically independent experiments. Scale bar 10 μm. g, h S2 cells overexpressing different combinations of the indicated proteins were grown on top of a ConA coated surface. YFP-Msk was immunoprecipitated with anti- GFP. The immunopurified complexes and total cell lysates (WCL) were immunoblotted as indicated. IgG was marked with an asterisk and Yki was marked with a #. Quantification from three biologically independent co-immunoprecipitations is shown (h). Statistical analysis with a two-tailed unpaired t-test. Data represent mean ± s.e.m. ns non-significant. P-value = 0.167 (4th vs. 5th bar), 0.00023 (4th vs. 6th bar) and 0.0029 (5th vs. 6th bar). Raw data are available in the Source Data file. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.

    Journal: Nature communications

    Article Title: Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP.

    doi: 10.1038/s41467-022-28693-y

    Figure Lengend Snippet: Fig. 6 Msk is required for Yki nuclear accumulation and Yki-induced organ growth in vivo. a, b Confocal images of Drosophila imaginal discs overexpressing GFP, GFP-Yki, and GFP-Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4). Quantification in the graph (b). From left to right, N = 7, 18, and 22 discs. Scale bar 100 μm. P-value = 2.870e−07 (column 1 vs. 2), 2.392e−06 (column 2 vs. 3) and 0.084 (column 1 vs. 3). c, d Confocal images of Drosophila imaginal discs overexpressing Yki and Yki + msk siRNA under the UAS/Gal4 system in the dorsal compartment. Quantification of Yki nucleo-cytoplasmic ratio is shown in graph (d). Zooms are shown below. N = 66 and 54 cells from a representative experiment of 3 independent experiments. Scale bar 10 μm. P-value = 1.007e−14. e Confocal images of Drosophila imaginal discs overexpressing Yki and stained against Yki and Msk. Zooms are shown below. Data representative from 3 biologically independent experiments. Scale bar 10 μm. f Confocal images of Drosophila imaginal discs overexpressing YFP-msk under the UAS/Gal4 system in the dorsal compartment (under the control of apterusGal4) and stained against Yki and Msk. Data representative of three biologically independent experiments. Scale bar 10 μm. g, h S2 cells overexpressing different combinations of the indicated proteins were grown on top of a ConA coated surface. YFP-Msk was immunoprecipitated with anti- GFP. The immunopurified complexes and total cell lysates (WCL) were immunoblotted as indicated. IgG was marked with an asterisk and Yki was marked with a #. Quantification from three biologically independent co-immunoprecipitations is shown (h). Statistical analysis with a two-tailed unpaired t-test. Data represent mean ± s.e.m. ns non-significant. P-value = 0.167 (4th vs. 5th bar), 0.00023 (4th vs. 6th bar) and 0.0029 (5th vs. 6th bar). Raw data are available in the Source Data file. P-values below or equal to 0.05, 0.01, or 0.005 were considered statistically significant and were labeled with 1, 2, or 3 asterisks, respectively.

    Article Snippet: S2 cells (ATCC, CRL-1963) were grown on Schneider ́s Drosophila medium (Gibco).

    Techniques: In Vivo, Control, Staining, Immunoprecipitation, Two Tailed Test, Labeling